How do Lectin Read the “Glyco Code”
Of all the building blocks of life, glycans are the most abundant and diverse family of organic molecules, which informational
Margherita Duca
Of all the building blocks of life, glycans are the most abundant and diverse family of organic molecules, which informational
Often possessing more than one binding site, lectins are thus intrinsically multivalent receptors that can interact with multiple copies of
When studying the role of lectin in biology or when assessing the capability of new therapeutics in (dis)regulating these functions,
Isothermal Titration Calorimetry (ITC) is considered the method of choice to analyse protein-carbohydrate interactions. It allows the precise determination of
Fluorescence polarization (FP) is a powerful tool in biochemical research. This method has historically been applied to the study of
In the early history of lectin research, the most common method to identify lectin activity was to check the ability
The enzyme-linked lectin assay (ELLA) was introduced in 1983 by McCoy et al. (. McCoy et al., 1983 ) as a variation
The phenomenon of surface plasmon resonance (SPR) was observed for the first time by Wood more than a century ago
The relevance of carbohydrate-lectin interactions in controlling many physiological processes is key to various disciplines. However, the understanding of the
Andre, S.; Pieters, R. J.; Vrasidas, I.; Kaltner, H.; Kuwabara, I.; Liu, F. T.; Liskamp, R. M. J.; Gabius, H.