Entitled “45 Years at Work with Glyco”, Prof. Hans Kamerling gave the opening lecture at the 13th European Training Course on Carbohydrates (Summer School Glycoscience) this April (2014) in Wageningen, The Netherlands. His lecture is a testimony to the evolution of glycoscience over 45 years of scientific endeavor, methodological and technological developments. The presentation includes scientific diagrams accompanied by photographs of pioneer glycoscientists who paved the way to established, mature areas and who are still innovating and revolutionising rapidly developing new fields. While providing an educational description of the evolution of the field, the series of pictures that constitutes this e-chapter is also replete of illustrations to be used in several educational actions.