Close this search box.

Glycosylated queuosines in tRNAs Optimize Translational Rate and Post-Embryonic Growth


X. Zhao, D. Ma, K. Ishiguro, H. Saito, S. Akichika, I. Matsuzawa, M. Mito, T. Irie , K. Ishibashi, , K. Wakabayashi, Y. Sakaguchi , T. Yokoyama , Y. Mishima, M. Shirouzu , S. Iwasaki, T. Suzuki , T. Suzuki


Cell, 2023 Nov 17:S0092-8674(23)01177-7., doi : 10.1016/j.cell.2023.10.026.

Transfer RNA (tRNA) modifications are critical for protein synthesis. Queuosine (Q), a 7-deaza-guanosine derivative, is present in tRNA anticodons. Q is further glycosylated with galactose and mannose to form galQ and manQ in vertebrate Tyr and Asp tRNAs. However, biogenesis and physiological relevance remain poorly understood. The authors report the biochemical identification of two RNA glycosylases, QTGAL and QTMAN, and the successful reconstitution of tRNA Q-glycosylation with nucleotide diphosphate sugars.

Q-glycosylation slowed elongation at the cognate codons UAC and GAC (GAU), respectively, according to ribosome profiling of knockout cells. The authors found that galactosylation of Q suppressed stop codon read-through. In addition, protein aggregates increased in cells lacking Q-glycosylation, suggesting that Q-glycosylation contributes to proteostasis. Cryo-EM of the human ribosome-tRNA complex revealed the molecular basis of codon recognition regulated by Q-glycosylation.

Latest news

In biological systems, vascular networks play a pivotal role in regulating the chemical compositions of...

Amylose, a linear polymer comprised of α-1,4-linked glucopyranose units, is renowned for its propensity to...

Algae play an important ecological role as oxygen producers and carbon sequesters and are the...

Streptococcus gordonii is a Gram-positive bacterial species that typically colonizes the human oral cavity, but...