Close this search box.

Glycoengineered Keratinocyte Library Reveals Essential Functions of Specific Glycans for all Stages of Herpes Simplex Virus-1 Infection


I. Bagdonaite, I.N. Marinova, A. M. Rudjord-Levann, E. M. H. Pallesen, S.L. King-Smith, R. Karlsson, T.B. Rømer, Y-H. Chen, R.L. Miller, S. Olofsson, R. Nordén , T. Bergström, S. Dabelsteen & H.H. Wandall


Nature Communications, 2023, 14 : 7000

Viral and host glycans represent an understudied aspect of host-pathogen interactions despite their potential implications for treating viral infections. This is due to the lack of readily available tools to analyse glycan function in a meaningful context. The authors generated a glycoengineered keratinocyte library of human glycosylation pathways to reveal the role of specific glycans at different stages of the herpes simplex virus type 1 (HSV-1) infection cycle. They demonstrate the importance of cellular glycosaminoglycans and glycosphingolipids for HSV-1 attachment for HSV-1 attachment, N-glycans for entry and spread, and O-glycans for replication.

While altered virion surface structures have minimal effects on the early interactions with wild-type cells, mutation of specific O-glycosylation sites affects glycoprotein surface expression and function. The data set demonstrates the importance of specific glycans in a clinically relevant human model of HSV-1 infection. It highlights the utility of genetic engineering to elucidate the roles of specific viral and cellular carbohydrate structures.

Latest news

The role of glycosaminoglycans (GAGs) in modulating bone morphogenetic protein (BMP) signaling is a relatively...

Glycans, due to their variable compositions and highly dynamic conformations, significantly enhance the diversity of...

Advances in simulation, combined with technological developments in high-performance computing, have made it possible to...

Antimicrobial resistance (AMR) is one of the most critical threats to global public health in...